An accurate, precise, sensitive, specific, robust, rugged simple and cost effective UV spectroscopic method has been developed for estimation of Ketoconazole (KZ) in bulk and tablet dosage form using methanol as a solvent. The degradation studies of KZ were studied as per guidelines of International Conference on Harmonisation (ICH). The λ max of KZ was found to be 203 nm. The method exhibited high accuracy and sensitivity, in a linear range of 2-7 µg/ml. The Limit of Detection (LOD) and Limit of Quantification (LOQ) were found to be 0.003 and 0.064 µg/ml respectively. All the concentrations were linear with the absorbance having a correlation coefficient 0.9956. The regression equation of calibration curve was found to be y = 0.1502x + 0.4168. The accuracy was found to be 99.17 ± 0.601026% with percentage recovery of 99.44 ± 0.35746%. Sample solution was stable for five hours, so that it could be concluded that the developed method would be applicable for the analysis for the KZ in bulk as well as in pharmaceutical formulations.
Loading....